The study design is "nested" within a prospective cohort where specimens and risk factors are available
Obviously, the nested case-control design is not feasible if there are not enough viable specimens available for analysis or risk factors of interest were not collected. Fresher specimens are preferred over long-term stored specimens because study results can be greatly altered based on the specimens themselves. However, from an empirical standpoint, the best part of a nested case-control study is that the recall bias associated with traditional case-control designs does not apply because the observations are analyzed prospectively.
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