The "I" in PICO represents the intervention, exposure, test, or agent
In between-subjects research designs, the predictor/independent variable is often related to group membership. In within-subjects designs, the intervention is given in between the baseline and post observations of the outcome. With multivariate designs, the association between the intervention and the outcome is adjusted for by demographic, clinical, prognostic, and confounding variables. The "I" or intervention in PICO is used by researchers to better understand outcomes.
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